Device

Part:BBa_K1088011:Design

Designed by: Andreas Kjær   Group: iGEM13_SDU-Denmark   (2013-08-28)


B. subtilis dxs (lac promoter without lac inhibitor)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1279
    Illegal EcoRI site found at 1936
    Illegal PstI site found at 1337
    Illegal PstI site found at 1783
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1279
    Illegal EcoRI site found at 1936
    Illegal PstI site found at 1337
    Illegal PstI site found at 1783
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1279
    Illegal EcoRI site found at 1936
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1279
    Illegal EcoRI site found at 1936
    Illegal PstI site found at 1337
    Illegal PstI site found at 1783
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1279
    Illegal EcoRI site found at 1936
    Illegal PstI site found at 1337
    Illegal PstI site found at 1783
    Illegal NgoMIV site found at 1236
    Illegal AgeI site found at 1129
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1078
    Illegal SapI.rc site found at 1777


Design Notes

We forgot to acknowledge the need for high expression of LacI to keep the regulation of the lac promoter vaild. The constructs where assembled with USER cloning, and therefore there are no scarsites between the parts. There are however inserted scarsites between the RBS and the coding region aswell as between the lac promoter and the RBS to improve translational efficiency


Source

The Lac promoter derives from E. coli. The Dxs protein is from B. sub

References